LABORATORY REPORT (Click on the Save a facsimile expiration on the panel above to save your report) bodily function: give: Instructor: Date: Enzyme Activity Khadija Mohamed Dr Smith declination 10, 2011 Predictions 1. invertase will lease the greatest legal serve at pH 6 2. invertase will have the greatest military action at 40 °C (104 °F) 3. Sucrase activity step-ups with change magnitude saccharose concentration until a plateau is reached. Materials and Methods found of pH on Enzyme Activity. 1. interdependent Variable. enumerate of product (glucose and fructose) produced 2. breakaway Variable. pH 3. Controlled Variables. temperature; amount of substrate ( sucrose) buy the farm over; invertase + sucrose brooding time Effect of Temperature on Enzyme Activity. 1. mutualist Variable. amount of product (glucose and fructose) produced 2. Independent Variable. temperature 3. Controlled Variables. pH; amount of substrate (sucrose) kick in; sacchar ase + sucrose incubation time Effect of Substrate Concentration on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. amount of substrate (sucrose) present 3. Controlled Variables. temperature; pH; sucrase + sucrose incubation time 1.
let on what is measured as an power of sucrase activity and why this is an indicator of sucrase activity. Glucose is measured as an indicator of sucrase as glucose is a product produced by sucrase activity. 2. explicate why denatured sucrase was used as a control. to determine if a genuine effect was do on the active sucrase by ph, temperatu re and sucrose or if fall would be seen on ! both denaturated and active sucrase 3. Alkaline DNS denatures sucrase. apologize why it is consequential to denature the enzyme. It is important to determine if ph, temperature and sucrose do have a true effect on active sucrase. leaving it active would increase the activity of the enzyme Results find across 1: Effect of pH on Sucrase Activity 1 2 3 average ph 3 0.1816 0.1873 0.1538 0.174 ph 4...If you want to get a full essay, order it on our website: BestEssayCheap.com
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